CD5L/AIM Regulates Lipid Biosynthesis and Restrains Th17 Cell Pathogenicity

نویسندگان

  • Chao Wang
  • Nir Yosef
  • Jellert Gaublomme
  • Chuan Wu
  • Youjin Lee
  • Clary B. Clish
  • Jim Kaminski
  • Sheng Xiao
  • Gerd Meyer Zu Horste
  • Mathias Pawlak
  • Yasuhiro Kishi
  • Nicole Joller
  • Katarzyna Karwacz
  • Chen Zhu
  • Maria Ordovas-Montanes
  • Asaf Madi
  • Ivo Wortman
  • Toru Miyazaki
  • Raymond A. Sobel
  • Hongkun Park
  • Aviv Regev
  • Vijay K. Kuchroo
چکیده

Th17 cells play a critical role in host defense against extracellular pathogens and tissue homeostasis but can induce autoimmunity. The mechanisms implicated in balancing "pathogenic" and "non-pathogenic" Th17 cell states remain largely unknown. We used single-cell RNA-seq to identify CD5L/AIM as a regulator expressed in non-pathogenic, but not in pathogenic Th17 cells. Although CD5L does not affect Th17 differentiation, it is a functional switch that regulates the pathogenicity of Th17 cells. Loss of CD5L converts non-pathogenic Th17 cells into pathogenic cells that induce autoimmunity. CD5L mediates this effect by modulating the intracellular lipidome, altering fatty acid composition and restricting cholesterol biosynthesis and, thus, ligand availability for Rorγt, the master transcription factor of Th17 cells. Our study identifies CD5L as a critical regulator of the Th17 cell functional state and highlights the importance of lipid metabolism in balancing immune protection and disease induced by T cells.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

CD5L/AIM regulates lipid biosythesis and restrains Th17 cell pathogenicity

Chao Wang1, Nir Yosef4,8, Jellert Gaublomme2,3, Chuan Wu1, Youjin Lee1, Clary B. Clish2, Jim Kaminski8, Sheng Xiao1, Gerd Meyer Zu Horste1, Mathias Pawlak1, Yasuhiro Kishi1,9, Nicole Joller1, Katarzyna Karwacz1, Chen Zhu1, Maria Ordovas-Montanes1, Asaf Madi1,2, Ivo Wortman2, Toru Miyazaki6, Raymond A. Sobel5, Hongkun Park2,3, Aviv Regev2,7, and Vijay K. Kuchroo1,2 1Evergrande Center for Immunol...

متن کامل

A Metabolic Switch for Th17 Pathogenicity

T helper 17 (Th17) cells are critical for host defense but can also drive autoimmunity. This divergent behavior is explored by Gaublomme et al. and Wang et al., who identify inflammation-associated genes by measuring gene expression in nearly 1,000 individual Th17 cells and show that CD5L affects the expression of pro-inflammatory genes by altering lipid synthesis.

متن کامل

Single-Cell Genomics Unveils Critical Regulators of Th17 Cell Pathogenicity

Extensive cellular heterogeneity exists within specific immune-cell subtypes classified as a single lineage, but its molecular underpinnings are rarely characterized at a genomic scale. Here, we use single-cell RNA-seq to investigate the molecular mechanisms governing heterogeneity and pathogenicity of Th17 cells isolated from the central nervous system (CNS) and lymph nodes (LN) at the peak of...

متن کامل

Genome-wide regulatory analysis reveals that T-bet controls Th17 lineage differentiation through direct suppression of IRF4.

The complex relationship between Th1 and Th17 cells is incompletely understood. The transcription factor T-bet is best known as the master regulator of Th1 lineage commitment. However, attention is now focused on the repression of alternate T cell subsets mediated by T-bet, particularly the Th17 lineage. It has recently been suggested that pathogenic Th17 cells express T-bet and are dependent o...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Cell

دوره 163  شماره 

صفحات  -

تاریخ انتشار 2015